Parallel Plate Flow Chamber Cell Culture

Planning setup and analysis experimental workflow before starting choosing the optimal setup cells under flow.

Parallel plate flow chamber cell culture.

The most common types of microchannels used in cell adhesion and flow studies are the radial flow chamber and the parallel plate flow chamber. Assemble flow system apparatus connecting inlet outlet and vacuum lines to the flow chamber deck. Although the here presented flow chamber follows a parallel plate design other chamber designs could be used as well. A parallel plate rheometer consists of two parallel discs one of which rotates.

Turn on stage incubator and warm adhesion media to 37 c 1 hr. Cells under flow the flow assay. To understand the influence of fluid shear and to. Several design modifications have occurred over the years yet a comparison of these different designs has not been performed.

The parallel plate flow chamber has become a mainstay for examination of leukocytes under physiologic flow conditions. On a more abstract level the device presents a platform that takes care of the well being of cells and the cassette like flow chamber creates an experimental environment that can be adjusted to individual needs. Flow assay using parallel plate flow chamber. Usually the torque is measured on the rotating disc.

Fill system with media and remove all air from system. Parallel plate flow chamber experiments with endothelial cells have shown that shear flow can align cells 50 143 144 145 146 147 148. Refer to protocol 7. 3 i used different microfluidic device designs with higher aspect ratio to make them more similar to the parallel plate flow chambers including the ones that have been published by others and.

Compatible with the ibidi heating systems all incubators incubated microscopes and channel µ slides. Dishes are ready for use in flow assay. Minimal mechanical stress minimal amount of medium and supplement needed. Various gasket sizes allow flexibility in experimental design to operate with limited quantities of cells and or reagents.

Endothelial cells under flow for simulation of blood vessels ideal simulation of various physiological conditions continuous unidirectional oscillating and pulsatile flow. In addition the reagent requirements of many designs prohibit the study of rare leukocyte populations and require large amounts of reagents. Parallel plate flow chambers are used in dynamic studies of cell adhesion under well defined shear forces. A pump system for the cultivation of e g.

There are radial membrane minichannels for hematopoietic blood cell culture the bioartificial liver membranes enhance mass transfer in planar microchannels and oxygen and lactate transport in micro. 3 2 1 parallel plate rheometer. In a parallel plate rheometer for a fluid such as oils or polymers the material is confined between the two shearing plates by capillary forces and does not flow out.